Validating affymetrix chip results with real time pcr updating white kitchen cabinets
However, the ranking was not affected by the normal assumption.
For the RT-PCR data, 1 technical replicate was arbitrarily chosen from each sample for the equivalence test.
One such analytic method in item response theory is the Rasch measurement, which is a way to convert ordinal observations into linearmeasures.
Current estimation strategies assume the independence of the Rasch model parameters.
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Publications listed below are automatically derived from MEDLINE/Pub Med and other sources, which might result in incorrect or missing publications.The assumption for this normalization is that the total signals from all studied ‘reference genes’ are the same across all samples.The preprocessing of the microarray data analysis was performed as previously described .Xiangqin Cui, Ph D and Michal Mrug, MDDivision of Nephrology, University of Alabama at Birmingham1900 University Blvd, Tinsley Harrison Tower 611JBirmingham, AL 35294 (USA)Tel.1 2, Fax 1 2, E-Mail [email protected]: Endogenous internal controls (‘reference’ or ‘housekeeping’ genes) are widely used in real-time PCR (RT-PCR) analyses.
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In contrast to the previously utilized 2-fold change threshold for the equivalence test , we used a more stringent 1.5-fold change threshold since a 2-fold change is often assumed to be differentially expressed and not appropriate to consider as a ‘reference gene’.